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mtPhyl is a free software tool for human mitochondrial DNA analysis and phylogeny reconstruction. The software was designed by Nikolay Eltsov and Natalia Volodko, two scientists working at the Laboratory of Human Molecular and Evolutionary Genetics in the Siberian Branch of the Russian Academy of Science. mtPhyl was initially designed to compare any mtDNA sequence or set of sequences with the revised Cambridge Reference Sequence, and then to export the results into Excel format. Subsequently, additional useful functions have been integrated. The current version of mtPhyl can perform a variety of different functions which include: analysing mutation features; exporting lists of mutations into an Excel table; defining mitochondrial haplotypes; calculating the coalescence time of clusters; and downloading complete human mtDNA sequences from GenBank.

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